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Revvity
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Evident Corporation
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Evident Corporation
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Evident Corporation
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Image Search Results
Journal: Cancer Medicine
Article Title: IGF2BP2 promotes pancreatic carcinoma progression by enhancing the stability of B3GNT6 mRNA via m6A methylation
doi: 10.1002/cam4.5096
Figure Lengend Snippet: IGF2BP2 regulates B3GNT6 mRNA expression to promote pancreatic carcinoma. (A) Prediction of IGF2BP2 target genes, Venn diagram shows substantial and significant overlap among TCGA‐GTEx, IGF2BP2‐CLIP and DEGs between PA1 and PA2 subgroups. (B) Expression of predicted genes after IGF2BP2 KO. C m6A IP and IGF2BP2 IP verified the binding efficacy of target genes. (D) TCGA‐GTEx reveals the high expression of B3GNT6 in PC. (E) Spearman correlation analysis reveals positive co‐expression between IGF2BP2 and B3GNT6. (F–H) CCK‐8 and EdU assay revealed the PC cell proliferation ability after B3GNT6 KO. (I) Transwell experiment revealed PC cell migration ability after B3GNT6 KO. (J) B3GNT6 KO inhibited colony formation ability in PC cells. (* p < 0.05).
Article Snippet: After dewaxing, hydration and antigen repair, paraffin sections were blocked with immunostaining blocking solution for 1 h, incubated with IGF2BP2 and
Techniques: Expressing, Binding Assay, CCK-8 Assay, EdU Assay, Migration
Journal: Cancer Medicine
Article Title: IGF2BP2 promotes pancreatic carcinoma progression by enhancing the stability of B3GNT6 mRNA via m6A methylation
doi: 10.1002/cam4.5096
Figure Lengend Snippet: IGF2BP2 regulates B3GNT6 mRNA stability via an m6A‐dependent manner and promotes m6A content in PC. (A) IGF2BP2 IP verified B3GNT6 expression after IGF2BP2 KO. (B‐C) Dual luciferase reporter assays verified the methylation of B3GNT6 by IGF2BP2. (D) B3GNT6 mRNA stability in cells treated with IGF2BP2‐si. (E) B3GNT6 protein expression in cells after treated with IGF2BP2‐si and 3‐DAA. (F–G) Immunofluorescence staining confirmed the co‐expression of IGF2BP2 and B3GNT6 in PC tissues (F) and cells (G). (H–J) Detection of the tumorigenic ability of PC cells, including tumor size and tumor weight. (K) Immunofluorescence staining confirmed the co‐expression of IGF2BP2 and B3GNT6 in mice tumor tissue. (L) IHC(B3GNT6)‐stained paraffin‐embedded sections verified the low expression of B3GNT6 protein after IGF2BP2 KO. (* p < 0.05).
Article Snippet: After dewaxing, hydration and antigen repair, paraffin sections were blocked with immunostaining blocking solution for 1 h, incubated with IGF2BP2 and
Techniques: Expressing, Luciferase, Methylation, Immunofluorescence, Staining
Journal: Cancer Medicine
Article Title: IGF2BP2 promotes pancreatic carcinoma progression by enhancing the stability of B3GNT6 mRNA via m6A methylation
doi: 10.1002/cam4.5096
Figure Lengend Snippet: Mechanism diagram of IGF2BP2 promoting B3GNT6 expression via m6A methylation.
Article Snippet: After dewaxing, hydration and antigen repair, paraffin sections were blocked with immunostaining blocking solution for 1 h, incubated with IGF2BP2 and
Techniques: Expressing, Methylation